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- IMAGEJ FOR WINDOWS WINDOWS 7
You can also use the arrow keys to move the rectangle, though this is slower. Use your mouse to click and hold in the middle of the rectangle on the 1st lane and drag it over to the next lane. The 1st lane will now be highlighted and have a 1 in the middle of it.ĥ. (Command + 1 on Mac) key (Command + 1 on Mac) or go to After drawing the rectangle over your first lane, press the The first lane has been selected with the Rectangular Selections toolĤ. Your image should look like Figure 1.įigure 2. The simplest method to convert to grayscale is to go to Image>Type>8-bit. The gel analysis routine requires the image to be a gray-scale image.
IMAGEJ FOR WINDOWS WINDOWS 7
This version of the tutorial was created using ImageJ 1.42q on a Windows 7 64-bit install.Ģ. There should be very little difference between the results obtained from the various methods. You may prefer to use it instead of the methods I outline below. The method outlined here uses the Gel Analysis method outlined in the ImageJ documentation: Gel Analysis. For an explanation of why overexposure will ruin your results, see this page. This also assumes that you didn’t overexposed your blot image when you produced it. See the references at the end of this tutorial for a discussion of the various ways that you can screw this step up.
IMAGEJ FOR WINDOWS SOFTWARE
film), and use the scanner software to save 16-bit tiff images.
If you are scanning x-ray film on a flatbed scanner, make sure you use a scanner with the ability to scan transparencies (i.e. tif would be the preferred format to retain the maximum amount of information in the original image). This tutorial assumes that you have carried your gel or blot through the visualization step, so that you have a digital image of your gel in. ImageJ ( ) can be used to compare the density (aka intensity) of bands on an agar gel or western blot.
IMAGEJ FOR WINDOWS FOR FREE
The Image Studio Lite software can be downloaded for free from In addition to the functionality described below for ImageJ, Image Studio Lite provides additional data management features along with annotation tools for producing publication-ready images of your westerns. If you’re looking for a more comprehensive workflow option for your western blot analyses, please visit my tutorial on using Image Studio Lite, a free software package from LI-COR Biosciences. Plant Proteases and Plant Cell Death: Methods and Protocols. Detection of Damage-Activated Metacaspase Activity by Western Blot in Plants. If you need a peer-reviewed citation for the methods outlined below, you may cite Stael, S., L.P. If the file size is more than 10MB consider removing slices or reducing the stack dimensions even further (step 5).The following information is an updated version of a method for using ImageJ to analyze western blots from a now-deprecated older page. For windows users FileOptimizer is good if a bit clunky. For an online optimisation tool use Ĩ.
IMAGEJ FOR WINDOWS FOR MAC
For mac users ImageOptim is excellent and free. Simply select Save As > Gif… from the file menu.ħ. Use an image optimisation tool to optimise the size of the GIF. Select Adjust > Size from the Image menu.Ħ. Resize the stack size if required – should be less than 1200px. If you want the animation slower or faster select Stacks > Animation > Animation Options… from the Image menu and change the frame rate.ĥ. Press the play button ‘>’ at the bottom left of the new stack to check the speed of the animation. Enter the slice numbers noted from step 2. From the Image menu then select Stacks > Tools > Make Substack…ģ.
Scroll through the stack using the left and right cursor keys and make a note of the slice number (shown at the top of the stack window) for slices you want to include in the animated GIF. More instructions for importing captured sequences can be found in the Processing Instructions.Ģ. Load the sequence of images into a stack using the Import > Image Sequence… option from the File menu. Aside from online tools such as there are also desktop applications like GIF Toaster for mac or GIF Maker for windows, as well as Adobe Photoshop, but the easiest way is to use ImageJ which you’ll already have installed for processing the images.ġ. There are a number of ways of creating animated GIFs for upload to the OpenSourceOV gallery.
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